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# raer <a href="https://rnabioco.github.io/raer"><img src="man/figures/logo.png" align="right" height="138" /></a>
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raer facilitates analysis of RNA adenosine editing in the
[Bioconductor](https://bioconductor.org/) ecosystem.
## Installation
`raer` is available on
[Bioconductor](https://bioconductor.org/packages/release/bioc/html/raer.html):
``` r
if (!require("BiocManager", quietly = TRUE)) {
install.packages("BiocManager")
}
BiocManager::install("raer")
```
You can install the development version of raer from
[GitHub](https://github.com/rnabioco/raer) with:
``` r
BiocManager::install("rnabioco/raer")
```
## Quick start
raer provides methods to compute per site read count summaries from BAM
alignment files, either for known editing sites, or for all detected
sites.
``` r
library(raer)
bam1fn <- raer_example("SRR5564269_Aligned.sortedByCoord.out.md.bam")
bam2fn <- raer_example("SRR5564277_Aligned.sortedByCoord.out.md.bam")
fafn <- raer_example("human.fasta")
bams <- c("ko" = bam1fn, "wt" = bam2fn)
rse <- pileup_sites(bams, fafn)
```
To facilitate comparisons across groups, base count data and genomic
coordinates are stored in a `RangedSummarizedExperiment`.
``` r
suppressMessages(library(SummarizedExperiment))
rse
#> class: RangedSummarizedExperiment
#> dim: 1695 2
#> metadata(0):
#> assays(7): ALT nRef ... nC nG
#> rownames(1695): site_SSR3_1_2 site_SSR3_2_2 ... site_DHFR_517_2
#> site_DHFR_518_2
#> rowData names(4): REF rpbz vdb sor
#> colnames(2): ko wt
#> colData names(1): sample
assays(rse)
#> List of length 7
#> names(7): ALT nRef nAlt nA nT nC nG
colData(rse)
#> DataFrame with 2 rows and 1 column
#> sample
#> <character>
#> ko ko
#> wt wt
```
``` r
assays(rse)$nRef[1:4, ]
#> ko wt
#> site_SSR3_1_2 13 12
#> site_SSR3_2_2 14 12
#> site_SSR3_3_2 14 12
#> site_SSR3_4_2 15 12
assays(rse)$nAlt[1:4, ]
#> ko wt
#> site_SSR3_1_2 0 0
#> site_SSR3_2_2 0 0
#> site_SSR3_3_2 0 0
#> site_SSR3_4_2 0 0
```
The `FilterParam()` class holds multiple options for customizing the
output of `pileup_sites()`.
``` r
fp <- FilterParam(
only_keep_variants = TRUE,
library_type = "fr-first-strand",
min_depth = 2
)
rse <- pileup_sites(bams, fafn, param = fp)
rse
#> class: RangedSummarizedExperiment
#> dim: 74 2
#> metadata(0):
#> assays(7): ALT nRef ... nC nG
#> rownames(74): site_SSR3_102_2 site_SSR3_125_2 ... site_DHFR_430_2
#> site_DHFR_513_2
#> rowData names(4): REF rpbz vdb sor
#> colnames(2): ko wt
#> colData names(1): sample
```
`pileup_cells()` provides support for quantifying editing sites in
single cell libraries.
``` r
scbam_fn <- raer_example("5k_neuron_mouse_possort.bam")
outdir <- tempdir()
editing_sites <- GRanges(
c(
"2:579:-",
"2:625:-",
"2:589:-"
),
REF = "A",
ALT = "G"
)
cbs <- c(
"CACCAAACAACAACAA-1",
"TATTCCACACCCTCTA-1",
"GACCTTCAGTTGTAAG-1"
)
sce <- pileup_cells(scbam_fn,
sites = editing_sites,
cell_barcodes = cbs,
param = fp,
output_directory = outdir
)
sce
#> class: SingleCellExperiment
#> dim: 3 3
#> metadata(0):
#> assays(2): nRef nAlt
#> rownames(3): site_2_579_2_AG site_2_589_2_AG site_2_625_2_AG
#> rowData names(2): REF ALT
#> colnames(3): CACCAAACAACAACAA-1 TATTCCACACCCTCTA-1 GACCTTCAGTTGTAAG-1
#> colData names(0):
#> reducedDimNames(0):
#> mainExpName: NULL
#> altExpNames(0):
```
``` r
assays(sce)$nRef
#> 3 x 3 sparse Matrix of class "dgCMatrix"
#> CACCAAACAACAACAA-1 TATTCCACACCCTCTA-1 GACCTTCAGTTGTAAG-1
#> site_2_579_2_AG 0 0 1
#> site_2_589_2_AG 1 1 2
#> site_2_625_2_AG 0 0 0
assays(sce)$nAlt
#> 3 x 3 sparse Matrix of class "dgCMatrix"
#> CACCAAACAACAACAA-1 TATTCCACACCCTCTA-1 GACCTTCAGTTGTAAG-1
#> site_2_579_2_AG 1 1 1
#> site_2_589_2_AG 0 0 0
#> site_2_625_2_AG 1 1 1
```
## Related work
Core routines in `raer` are implemented using the `htslib` library and
methods from `samtools` and `bcftools`. `raer` builds off of approaches
from other RNA editing detection tools:
- [REDItools](https://github.com/BioinfoUNIBA/REDItools) from [Picardi
E, Pesole G](https://doi.org/10.1093/bioinformatics/btt287)
- [JACUSA2](https://github.com/dieterich-lab/JACUSA2) from [Piechotta M
et al](https://doi.org/10.1186/s12859-016-1432-8)
- [deNovo-Detect](https://github.com/a2iEditing/deNovo-Detect) from
[Gabay O et al](https://doi.org/10.1038/s41467-022-28841-4)
- [RNAEditingIndexer](https://github.com/a2iEditing/RNAEditingIndexer)
from [Roth SH et al](https://doi.org/10.1038/s41592-019-0610-9)
- [SAILOR](https://github.com/YeoLab/sailor) from [Washburn MC et
al](https://10.1016/j.celrep.2014.01.011)
