Bioconductor Code: multicrispr

Name	Mode	Size
R	040000
inst	040000
man	040000
public	040000
tests	040000
vignettes	040000
.Rbuildignore	100644	0 kb
.gitignore	100644	0 kb
DESCRIPTION	100644	2 kb
NAMESPACE	100644	3 kb
NEWS	100644	0 kb
README.md	100644	2 kb
_pkgdown.yml	100644	0 kb

README.md

# multicrispr: gRNA design [![](https://bioconductor.org/shields/build/devel/bioc/multicrispr.svg)](http://bioconductor.org/checkResults/devel/bioc-LATEST/multicrispr/) [![](https://bioconductor.org/shields/availability/3.12/multicrispr.svg)](https://bioconductor.org/packages/devel/bioc/html/multicrispr.html#archives) [![](https://bioconductor.org/shields/years-in-bioc/multicrispr.svg)](https://bioconductor.org/packages/devel/bioc/html/multicrispr.html#since) [![](https://img.shields.io/badge/doi-10.26508/lsa.202000757-blue.svg)](https://doi.org/10.26508/lsa.202000757) ### Installation # Install multicrispr remotes::install_git('https://gitlab.gwdg.de/loosolab/software/multicrispr.git', repos = BiocManager::repositories()) # Install azimuth install.packages('reticulate') reticulate::conda_create('azienv', c('python=2.7')) reticulate::use_condaenv('azienv', required=TRUE) reticulate::py_install(c('azimuth', 'scikit-learn==0.17.1', 'biopython==1.76'), 'azienv', pip = TRUE) # Index mm10 and hg38 BiocManager::install('BSgenome.Mmusculus.UCSC.mm10') BiocManager::install('BSgenome.Hsapiens.UCSC.hg38') multicrispr::index_genome(BSgenome.Mmusculus.UCSC.mm10::BSgenome.Mmusculus.UCSC.mm10) multicrispr::index_genome(BSgenome.Hsapiens.UCSC.hg38::BSgenome.Hsapiens.UCSC.hg38) ### Overview ![](https://gitlab.gwdg.de/loosolab/software/multicrispr/-/wikis/uploads/cdf31586bcf776a7a40acaaaf5172e10/overview.png)