# demuxmix demuxmix is a package for demultiplexing single-cell sequencing experiments of pooled cells labeled with barcode oligonucleotides. The package implements methods to fit regression mixture models for a probabilistic classification of cells, including multiplet detection. Demultiplexing error rates can be estimated, and methods for quality control are provided. ## Installation The package is available at [Bioconductor](https://bioconductor.org) and can be installed via BiocManager::install: ``` r if (!require("BiocManager")) install.packages("BiocManager") BiocManager::install("demuxmix") ``` The package only needs to be installed once. Load the package into an R session with ``` r library(demuxmix) ``` ## Vignette Please consult the package vignette for example usages of the methods in demuxmix. The vignette can be accessed by entering the following command and clicking on the resulting HTML link: ``` r browseVignettes("demuxmix") ``` ## Manuscript A manuscript describing the technical details of demuxmix is available at Bioinformatics: Hans-Ulrich Klein, demuxmix: demultiplexing oligonucleotide-barcoded single-cell RNA sequencing data with regression mixture models, *Bioinformatics*, Volume 39, Issue 8, August 2023, btad481 <https://doi.org/10.1093/bioinformatics/btad481>