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[![Build Status](]( # dada2 Exact sample inference from high-throughput amplicon data. Resolves real variants differing by as little as one nucleotide. Visit [the DADA2 website]( for the most detailed and up-to-date documentation. ### Installation The dada2 package binaries are available through Bioconductor: ```S ## try http:// if https:// URLs are not supported if (!requireNamespace("BiocManager", quietly=TRUE)) install.packages("BiocManager") BiocManager::install("dada2") ``` In order to install dada2 from source (and get the latest and greatest new features) see our [installation from source instructions]( ### Documentation The [tutorial walkthrough of the DADA2 pipeline on paired end Illumina Miseq data]( The [dada2 R package manual]( Further documentation is available on [the DADA2 front page]( ### DADA2 Articles [DADA2: High resolution sample inference from Illumina amplicon data. Nature Methods, 2016.]( [(Open Access link.)]( [Bioconductor workflow for microbiome data analysis: from raw reads to community analyses. F1000 Research, 2016.]( [Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISMEJ, 2017.]( [High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Research, 2019.]( ### Other Resources Planned feature improvements are publicly catalogued at the main DADA2 development site on github, specifically on the "Issues" page for DADA2: If the feature you are hoping for is not listed, you are welcome to add it as a feature request "issue" on this page. This request will be publicly available and listed on the page. Bugs and difficulties in using DADA2 are also welcome on [the issue tracker](