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README.md
# _wiggleplotr_ _wiggleplotr_ is a tool to visualise RNA-seq read overage overlapping gene annotations. A key feature of _wiggleplotr_ is that it is able rescale all introns of a gene to fixed length, making it easier to see differences in read coverage between neighbouring exons that can otherwise be too far away. Since _wiggleplotr_ takes standard BigWig files as input, it can also be used to visualise read overage from other sequencing-based assays such as ATAC-seq and ChIP-seq. <img src="PTK2B.png" width="450"> ## Installation _wiggleplotr_ is soon going to be submitted to Bioconductor, but in the mean time it can be installed directly from GitHub: ```r library("devtools") devtools::install_github("kauralasoo/wiggleplotr") ``` ## Getting started See the [vignette](https://htmlpreview.github.io/?https://github.com/kauralasoo/wiggleplotr/blob/master/vignettes/wiggleplotr.html) for instructions on how to get started. ## Citation If you use this software for research, please cite the following paper in which it was first described: [Transcriptional profiling of macrophages derived from monocytes and iPS cells identifies a conserved response to LPS and novel alternative transcription (Alasoo et al, 2015)](http://www.nature.com/articles/srep12524)