... | ... |
@@ -338,11 +338,20 @@ on this to run the cell type labeling process.</li> |
338 | 338 |
annotation will be inserted to the background object. They will all have |
339 | 339 |
the same prefix as |
340 | 340 |
<code>"SingleR_{reference abbr}_{annotation level}_"</code>, then |
341 |
+<code>"score"</code>, <code>"labels"</code>, <code>"delta.next"</code> |
|
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+and <code>"pruned.labels"</code>, respectively. <code>"labels"</code> |
|
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+contains the predicted label, basing only on the maximum entry in |
|
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+scores. <code>"delta.next"</code> contains difference between the best |
|
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+and next-best score. <code>"pruned.labels"</code> contains predictions |
|
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+where “low-quality” labels are replaced with <code>NA</code>s.</p> |
|
347 |
+<blockquote> |
|
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+<p>With older versions of SingleR, the output variables ends with |
|
341 | 349 |
<code>"score"</code>, <code>"first.labels"</code>, <code>"labels"</code> |
342 | 350 |
and <code>"pruned.labels"</code>, respectively. |
343 | 351 |
<code>"first.labels"</code> refers to the labeling initially indicated |
344 | 352 |
by the scores, <code>"labels"</code> is fine-tuned, and |
345 | 353 |
<code>"pruned.labels"</code> is the pruned result.</p> |
354 |
+</blockquote> |
|
346 | 355 |
<p><strong>Visualization</strong></p> |
347 | 356 |
<p>SCTK does not have a quick visualization at this point. But users can |
348 | 357 |
go to the <a href="visualization.html">CellViewer</a> tab to create a |
... | ... |
@@ -384,6 +393,14 @@ contains the predicted label, basing only on the maximum entry in |
384 | 393 |
scores. <code>"delta.next"</code> contains difference between the best |
385 | 394 |
and next-best score. <code>"pruned.labels"</code> contains predictions |
386 | 395 |
where “low-quality” labels are replaced with <code>NA</code>s.</p> |
396 |
+<blockquote> |
|
397 |
+<p>With older versions of SingleR, the output variables ends with |
|
398 |
+<code>"score"</code>, <code>"first.labels"</code>, <code>"labels"</code> |
|
399 |
+and <code>"pruned.labels"</code>, respectively. |
|
400 |
+<code>"first.labels"</code> refers to the labeling initially indicated |
|
401 |
+by the scores, <code>"labels"</code> is fine-tuned, and |
|
402 |
+<code>"pruned.labels"</code> is the pruned result.</p> |
|
403 |
+</blockquote> |
|
387 | 404 |
<p>Besides, users can also use their own labeled reference datasets |
388 | 405 |
wrapped in a <a href="https://rdrr.io/bioc/SingleCellExperiment/man/SingleCellExperiment.html" class="external-link">SingleCellExperiment</a> |
389 | 406 |
object. Refer to argument <code>useSCERef</code> and |
... | ... |
@@ -62,7 +62,9 @@ There are 6 basic widgets that users need to work with before reaching to the re |
62 | 62 |
|
63 | 63 |
6. **Label** - The button to trigger the algorithm. Click on this to run the cell type labeling process. |
64 | 64 |
|
65 |
-The result will not be pop out directly, but three levels of cell annotation will be inserted to the background object. They will all have the same prefix as `"SingleR_{reference abbr}_{annotation level}_"`, then `"score"`, `"first.labels"`, `"labels"` and `"pruned.labels"`, respectively. `"first.labels"` refers to the labeling initially indicated by the scores, `"labels"` is fine-tuned, and `"pruned.labels"` is the pruned result. |
|
65 |
+The result will not be pop out directly, but three levels of cell annotation will be inserted to the background object. They will all have the same prefix as `"SingleR_{reference abbr}_{annotation level}_"`, then `"score"`, `"labels"`, `"delta.next"` and `"pruned.labels"`, respectively. `"labels"` contains the predicted label, basing only on the maximum entry in scores. `"delta.next"` contains difference between the best and next-best score. `"pruned.labels"` contains predictions where "low-quality" labels are replaced with `NA`s. |
|
66 |
+ |
|
67 |
+> With older versions of SingleR, the output variables ends with `"score"`, `"first.labels"`, `"labels"` and `"pruned.labels"`, respectively. `"first.labels"` refers to the labeling initially indicated by the scores, `"labels"` is fine-tuned, and `"pruned.labels"` is the pruned result. |
|
66 | 68 |
|
67 | 69 |
**Visualization** |
68 | 70 |
|
... | ... |
@@ -96,6 +98,8 @@ sce <- runSingleR(inSCE = sce, useAssay = "logcounts", useBltinRef = "hpca", lev |
96 | 98 |
|
97 | 99 |
Four results from SingleR will be stored in `colData()` slot with the same prefix as `"SingleR_{reference abbr}_{annotation level}_"`, then `"score"`, `"labels"`, `"delta.next"` and `"pruned.labels"`, respectively. `"labels"` contains the predicted label, basing only on the maximum entry in scores. `"delta.next"` contains difference between the best and next-best score. `"pruned.labels"` contains predictions where "low-quality" labels are replaced with `NA`s. |
98 | 100 |
|
101 |
+> With older versions of SingleR, the output variables ends with `"score"`, `"first.labels"`, `"labels"` and `"pruned.labels"`, respectively. `"first.labels"` refers to the labeling initially indicated by the scores, `"labels"` is fine-tuned, and `"pruned.labels"` is the pruned result. |
|
102 |
+ |
|
99 | 103 |
Besides, users can also use their own labeled reference datasets wrapped in a [SingleCellExperiment](https://rdrr.io/bioc/SingleCellExperiment/man/SingleCellExperiment.html) object. Refer to argument `useSCERef` and `labelColName`. Additionally, the labeling can also be done on cluster label if users have already performed clustering on their dataset and have the result stored in `colData()` slot. Refer to argument `labelByCluster`. |
100 | 104 |
|
101 | 105 |
**Visualization** |