# rawrr The package provides access to proprietary Thermo Fisher Scientific Orbitrap instrument data as a stand-alone R package or serves as [MsRawFileReaderBackend](https://github.com/cpanse/MsBackendRawFileReader) for the Bioconductor [Spectra](https://bioconductor.org/packages/Spectra/) package. rawrr wraps the functionality of the [RawFileReader](https://planetorbitrap.com/rawfilereader) .NET assembly. Test files are provided by the [tartare](https://bioconductor.org/packages/tartare/) ExperimentData package. ## Install ### build package Please install the latest release from https://github.com/fgcz/rawR/releases according to the provided instructions. ### from source * install https://planetorbitrap.com/rawfilereader DLL files, e.g., copy file to `/usr/local/lib/RawFileReader/` * set `export MONO_PATH=/usr/local/lib/RawFileReader/` ## System requirements * [R](https://cran.r-project.org/) (>= 4.1) * [New RawFileReader .Net Assembly from Thermo Fisher Scientific](https://planetorbitrap.com/rawfilereader) * U.S. language setting ### MS Windows Make sure that .NET Framework 4.5.1 or higher is installed. ### macOS|Linux You need to have [Mono](https://www.mono-project.com) installed. Mono is an open source implementation of Microsoft's .NET Framework. ## Manuscripts https://www.biorxiv.org/content/10.1101/2020.10.30.362533v1 (accepted at [ACS JPR](https://pubs.acs.org/journal/jprobs)) ## Blog posts http://proteomicsnews.blogspot.com/2020/11/raw-mass-spec-data-is-too-pretty-for.html ## Slides MsRawFileReaderBackend presentaton at [European Biocondutor Meeting, de Duve Institute, UCLouvain, Campus de Woluw ́e Brussels, Belgium, Dec 2019](http://fgcz-ms.uzh.ch/~cpanse/talks/rawR_EuroBioc2019_Brussels_88c9.pdf)