Package: pram Title: Pooling RNA-seq datasets for assembling transcript models Version: 1.19.0 Authors@R: c( person( given="Peng", family="Liu", email = "pliu55.wisc@gmail.com", role = c("aut", "cre")), person( given="Colin N.", family="Dewey", role = c("aut")), person( given="Sündüz", family="Keleş", role = c("aut")) ) Description: Publicly available RNA-seq data is routinely used for retrospective analysis to elucidate new biology. Novel transcript discovery enabled by large collections of RNA-seq datasets has emerged as one of such analysis. To increase the power of transcript discovery from large collections of RNA-seq datasets, we developed a new R package named Pooling RNA-seq and Assembling Models (PRAM), which builds transcript models in intergenic regions from pooled RNA-seq datasets. This package includes functions for defining intergenic regions, extracting and pooling related RNA-seq alignments, predicting, selected, and evaluating transcript models. License: GPL (>= 3) Encoding: UTF-8 LazyData: true URL: https://github.com/pliu55/pram BugReports: https://github.com/pliu55/pram/issues Depends: R (>= 3.6) Imports: methods, BiocParallel, tools, utils, data.table (>= 1.11.8), GenomicAlignments (>= 1.16.0), rtracklayer (>= 1.40.6), BiocGenerics (>= 0.26.0), GenomeInfoDb (>= 1.16.0), GenomicRanges (>= 1.32.0), IRanges (>= 2.14.12), Rsamtools (>= 1.32.3), S4Vectors (>= 0.18.3) RoxygenNote: 6.1.0 Suggests: testthat, BiocStyle, knitr, rmarkdown Collate: 'Param.R' 'Transcript.R' 'buildModel.R' 'defIgRanges.R' 'evalModel.R' 'prepIgBam.R' 'runPRAM.R' 'selModel.R' 'util.R' VignetteBuilder: knitr biocViews: Software, Technology, Sequencing, RNASeq, BiologicalQuestion, GenePrediction, GenomeAnnotation, ResearchField, Transcriptomics SystemsRequirements: buildModel() and runPRAM() functions require external software Cufflinks, StringTie, and/or TACO. For details, please see the 'Required external software' section in vignette's 'Building transcript models: buildModel()'.