## use estimated positional bias profiles for fragment selection ### RNA fragmentation model: rnaf = read.csv('rnaf_pos_bias.csv', header=FALSE, colClasses=c('numeric', 'numeric')) names(rnaf) = c('pospct', 'prob') rnaf$pospct = seq(0.01, 1, by=0.01) #x-axis should be calibrated from 0-99%, but it was hard to click exactly :) rnaf$prob[rnaf$prob < 0] = 0 #minor adjustment to y-axis calibration # make sure probabilities sum to 1: rnaf$prob = rnaf$prob/sum(rnaf$prob) #it was super close anyway, but let's be careful. save(rnaf, file='data/rnaf.rda', compress='xz') ### cDNA fragmentation model: cdnaf = read.csv('cdna_pos_bias.csv', header=FALSE, colClasses=c('numeric', 'numeric')) names(cdnaf) = c('pospct', 'prob') cdnaf$pospct = seq(0.01, 1, by=0.01) cdnaf$prob[cdnaf$prob < 0] = 0 cdnaf$prob = cdnaf$prob/sum(cdnaf$prob) save(cdnaf, file='data/cdnaf.rda', compress='xz')