test_gsnap_syscall_unique_only_TRUE <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = TRUE, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) ##"unique_only=TRUE" means npath=1, no_fails is true, and ##split_output is false pieces <- unlist(strsplit(systemCall, " ")) checkTrue(any(pieces == "--npaths=1")) checkTrue(any(pieces == "--nofails")) checkTrue(!any(pieces == "split-output")) } test_gsnap_syscall_unique_only_FALSE <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = FALSE, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) ##"unique_only=FALSE" means npath=100L, no_fails is false, and ##split_output is true pieces <- unlist(strsplit(systemCall, " ")) ##since npaths=100 is a default, "npaths" should not be in the ##command line checkTrue(!any(grepl("--npaths=", pieces))) checkTrue(!any(pieces == "--nofails")) checkTrue(sum(grepl("^--split-output=", pieces)) == 1) } test_gsnap_syscall_max_mismatches <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = FALSE, gunzip=TRUE, max_mismatches=8) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue(any(pieces == "--max-mismatches=8")) } test_gsnap_syscall_suboptimal_levels <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = FALSE, gunzip=TRUE, suboptimal_levels=3) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue(any(pieces == "--suboptimal-levels=3")) } test_gsnap_syscall_suboptimal_levels_default <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = FALSE, gunzip=TRUE, suboptimal_levels=0) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue(!any(grepl("^--suboptimal-levels=", pieces))) } test_gsnap_mode_incorrect <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, gunzip=TRUE, mode="cmet") checkException(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) } test_gsnap_syscall_mode <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, gunzip=TRUE, mode="cmet-stranded") systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue(any(pieces == "--mode=cmet-stranded")) } test_gsnap_syscall_novelsplicing_FALSE <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, novelsplicing = FALSE, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue(sum(grepl("^--novelsplicing=", pieces)) == 0) } test_gsnap_syscall_novelsplicing_TRUE <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, unique_only = FALSE, max_mismatches = NULL, suboptimal_levels = 0, mode = "standard", npaths = 10, novelsplicing = TRUE, splicing = NULL, nthreads = 1, batch = 2L, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue("--novelsplicing=1" %in% pieces) } test_gsnap_syscall_nthreads_TRUE <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, nthreads=8, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue("--nthreads=8" %in% pieces) } test_gsnap_syscall_part <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, part="3/99", gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue("--part=3/99" %in% pieces) } test_gsnap_syscall_batch <- function() { gmapGenome <- TP53Genome() fastqs <- LungCancerLines::LungCancerFastqFiles() gsnapParam <- GsnapParam(genome = gmapGenome, batch=4, gunzip=TRUE) systemCall <- gmapR:::asSystemCall(gsnap(input_a=fastqs["H1993.first"], input_b=fastqs["H1993.last"], params=gsnapParam)) pieces <- unlist(strsplit(systemCall, " ")) checkTrue("--batch=4" %in% pieces) }