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### =========================================================================
### gsnap command
### -------------------------------------------------------------------------
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### High-level interface
###
setGeneric("gsnap", function(input_a, input_b = NULL, params, ...)
standardGeneric("gsnap"))
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setMethod("gsnap", c("character", "character_OR_NULL", "GsnapParam"),
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function(input_a, input_b, params,
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output = file.path(getwd(),
file_path_sans_ext(basename(input_a), TRUE)),
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consolidate = TRUE, ...)
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{
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if (!is.null(input_b) && length(input_a) != length(input_b))
stop("If 'input_b' is non-NULL, it must have the same length",
" as 'input_a'")
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if (anyNA(input_a) || anyNA(input_b))
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stop("'input_a' and 'input_b' must not contain NA's")
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if (length(input_a) > 1L) {
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args <- list(params, consolidate, ...)
return(GsnapOutputList(mapply(gsnap, input_a, input_b,
output=output,
MoreArgs = args)))
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}
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output_dir <- dirname(output)
if (!file.exists(output_dir))
dir.create(output_dir, recursive = TRUE)
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params <- initialize(params, ...)
params_list <- as.list(params)
if (gsnap_split_output(params)) {
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params_list$split_output <- output
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output_path <- output_dir
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} else {
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output_path <- paste0(output, ".sam")
params_list$.redirect <- paste(">", output_path)
}
if (is.null(params_list$gunzip)) {
if (file_ext(input_a) == "gz" && file_ext(input_b) == "gz") {
params_list$gunzip <- TRUE
}
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}
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res <- do.call(.gsnap,
c(list(.input_a = input_a, .input_b = input_b,
format = "sam"),
params_list))
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gsnap_output <- GsnapOutput(path = output_path,
version = gsnapVersion(),
param = params)
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gsnap_output <- asBam(gsnap_output)
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if (consolidate)
consolidate(gsnap_output)
return(gsnap_output)
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})
### - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
### Low-level interface
###
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.gsnap <- function(db = NULL, dir = NULL, part = NULL,
input_buffer_size = 1000L,
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barcode_length = 0L,
orientation = c("FR", "RF", "FF"),
fastq_id_start = NULL, fastq_id_end = NULL,
filter_chastity = c("off", "either", "both"),
gunzip = FALSE,
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batch = c("2", "0", "1", "3", "4"), max_mismatches = NULL,
query_unk_mismatch = 0L, genome_unk_mismatch = 1L,
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terminal_threshold = 2L,
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indel_penalty = 2L,
indel_endlength = 4L, max_middle_insertions = 9L,
max_middle_deletions = 30L, max_end_insertions = 3L,
max_end_deletions = 6L, suboptimal_levels = 0L,
adapter_string = NULL,
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trim_mismatch_score = -3L, trim_indel_score = -4L,
snpsdir = NULL, use_snps = NULL,
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cmetdir = NULL, atoidir = NULL,
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mode = c("standard", "cmet-stranded", "cmet-nonstranded",
"atoi-stranded", "atoi-nonstranded"),
tallydir = NULL, use_tally = NULL,
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use_runlength = NULL, nthreads = 1L,
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gmap_mode = "pairsearch,terminal,improve",
trigger_score_for_gmap = 5L, max_gmap_pairsearch = 3L,
max_gmap_improvement = 3L, microexon_spliceprob = 0.90,
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novelsplicing = 0L, splicingdir = NULL,
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use_splicing = NULL, ambig_splice_noclip = FALSE,
localsplicedist = 200000L,
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local_splice_penalty = 0L, distant_splice_penalty = 3L,
distant_splice_endlength = 16L,
shortend_splice_endlength = 2L,
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distant_splice_identity = 0.95,
antistranded_penalty = 0L, merge_distant_samechr = FALSE,
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pairmax_dna = 1000L,
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pairmax_rna = 200000L, pairexpect = 200L, pairdev = 25L,
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quality_protocol = c("sanger", "illumina"),
quality_zero_score = 33L, quality_print_shift = 0L,
mapq_unique_score = NULL, npaths = 100L,
quiet_if_excessive = FALSE, ordered = FALSE,
show_rediff = FALSE, clip_overlap = FALSE,
print_snps = FALSE, failsonly = FALSE,
nofails = FALSE, fails_as_input = FALSE,
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format = NULL, split_output = NULL,
output_buffer_size = 1000L,
no_sam_headers = FALSE,
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sam_headers_batch = NULL, read_group_id = NULL,
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read_group_name = NULL, read_group_library = NULL,
read_group_platform = NULL, version = FALSE,
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.input_a = NULL, .input_b = NULL,
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.redirect = NULL ## e.g., > gsnap.sam
)
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{
formals <- formals(sys.function())
problems <- do.call(c, lapply(names(formals), .valid_gmap_parameter, formals))
if (!is.null(problems))
stop("validation failed:\n ", paste(problems, collapse = "\n "))
orientation <- match.arg(orientation)
batch <- match.arg(batch)
mode <- match.arg(mode)
quality_protocol <- match.arg(quality_protocol)
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filter_chastity <- match.arg(filter_chastity)
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if (version) {
.redirect <- ">/dev/null"
}
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### TODO: if input_a is NULL, or split_output and .redirect are NULL:
### return a pipe()
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.system_gsnap(commandLine("gsnap"))
}
..gsnap <- function(args, path = NULL) {
.system_gsnap(.commandLine("gsnap", args, path))
}
.system_gsnap <- function(command) {
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stderr <- tempfile("gsnap-stderr", fileext = "log")
command <- paste(command, "2>", stderr)
.system(command)
output <- readLines(stderr)
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if (!gsnapSucceeded(command, output))
stop("Execution of gsnap failed, command-line: '", command,
"'; last output line: '", tail(output, 1), "'")
else output
}
gsnapSucceeded <- function(command, output) {
if (!grepl("--version", command))
any(grepl("^Processed \\d+ queries", output))
else TRUE
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}
### - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
### Validity Checking
###
.valid_GsnapParam_part <- function(x) {
## validate the part string (i/n)
}
.valid_GsnapParam_batch <- function(x) {
## one of 0, 1, 2, 3, 4
}
.valid_GsnapParam_max_mismatches <- function(x) {
## non-negative number or NULL
}
.valid_GsnapParam_suboptimal_levels <- function(x) {
## non-negative number or NULL
}
.valid_GsnapParam_use_snps <- function(x) {
## a file that exists (not sure)
}
.valid_GsnapParam_mode <- function(x) {
## one of standard, cmet, atoi
}
.valid_GsnapParam_nthreads <- function(x) {
## positive number
}
.valid_GsnapParam_novelsplicing <- function(x) {
## TRUE or FALSE
}
.valid_GsnapParam_splicing <- function(x) {
## a file that exists (not sure) or NULL
}
.valid_GsnapParam_npaths <- function(x) {
## positive number
}
.valid_GsnapParam_quiet_if_excessive <- function(x) {
## TRUE or FALSE
}
.valid_GsnapParam_nofails <- function(x) {
## TRUE or FALSE
}
.valid_GsnapParam_format <- function(x) {
## sam or NULL (this is a low-level check!)
}
.valid_GsnapParam_split_output <- function(x) {
## single string or NULL
}
.valid_GsnapParam_read_group_id <- function(x) {
## single string (any character constraints?) or NULL
}
.valid_GsnapParam_read_group_name <- function(x) {
## single string or NULL
}
.valid_gmap_parameter <- function(name, params) {
res <- TRUE ##if we're not validating the param, assume it is good
validatorName <- paste(".valid_GsnapParam_", name, sep = "")
if (exists(validatorName)) {
validator <- get(validatorName)
res <- validator(params)
} else {
res <- NULL
}
return(res)
}
.valid_GsnapParam <- function(object) {
x <- as.list(object) # converts to low-level parameter list
do.call(c, lapply(names(x), .valid_gmap_parameter, x))
}
setValidity("GsnapParam", .valid_GsnapParam)
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### Utilities
###
gsnapVersion <- function() {
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output <- .gsnap(version = TRUE)
version_text <- sub("GSNAP version (.*?) .*", "\\1", output[1])
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parseGsnapVersion(version_text)
}
parseGsnapVersion <- function(x) {
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as.POSIXlt(x, format = "%Y-%m-%d")
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}
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