\name{read.spot}
\description{
Read all file, but only extract the interested columns and create a Spot object.
}
\usage{
FALSE, sep = "\t", is.ifc = FALSE, envir)
}
\arguments{
\item{file.name}{a connection or a character string giving the
name of the file to read where each column represent the spot components.}
\item{cy3}{column that represent Cy3.}
\item{cy5}{column that represent Cy5.}
\item{bg.cy3}{column that represent BgCy3.}
\item{bg.cy5}{column that represent BgCy5.}
\item{ids}{column that represent Id.}
\item{symdesc}{(optional) identifier besides Id column.}