Package: deepSNV Maintainer: Moritz Gerstung <moritz.gerstung@ebi.ac.uk> License: GPL-3 Title: Detection of subclonal SNVs in deep sequencing data. biocViews: GeneticVariability, SNP, Sequencing, Genetics, DataImport LinkingTo: Rhtslib (>= 1.13.1) Type: Package LazyLoad: yes Authors@R: c( person("Niko","Beerenwinkel", role="ths"), person("Raul", "Alcantara", role="ctb"), person("David", "Jones", role = "ctb"), person("John", "Marshall", role = "ctb"), person("Inigo", "Martincorena", role = "ctb"), person("Moritz","Gerstung", email = "moritz.gerstung@ebi.ac.uk", role= c("aut","cre")) ) Description: This package provides provides quantitative variant callers for detecting subclonal mutations in ultra-deep (>=100x coverage) sequencing experiments. The deepSNV algorithm is used for a comparative setup with a control experiment of the same loci and uses a beta-binomial model and a likelihood ratio test to discriminate sequencing errors and subclonal SNVs. The shearwater algorithm computes a Bayes classifier based on a beta-binomial model for variant calling with multiple samples for precisely estimating model parameters - such as local error rates and dispersion - and prior knowledge, e.g. from variation data bases such as COSMIC. Version: 1.49.0 Depends: R (>= 2.13.0), methods, graphics, parallel, IRanges, GenomicRanges, SummarizedExperiment, Biostrings, VGAM, VariantAnnotation (>= 1.27.6), Imports: Rhtslib Suggests: RColorBrewer, knitr, rmarkdown VignetteBuilder: knitr SystemRequirements: GNU make RoxygenNote: 7.0.2