% Generated by roxygen2: do not edit by hand % Please edit documentation in R/perplexity.R \name{resamplePerplexity} \alias{resamplePerplexity} \alias{resamplePerplexity,SingleCellExperiment-method} \alias{resamplePerplexity,matrix-method} \title{Calculate and visualize perplexity of all models in a celdaList, with count resampling} \usage{ resamplePerplexity(x, ...) \S4method{resamplePerplexity}{SingleCellExperiment}( x, useAssay = "counts", altExpName = "featureSubset", resample = 5, seed = 12345 ) \S4method{resamplePerplexity}{matrix}(x, celdaList, resample = 5, seed = 12345) } \arguments{ \item{x}{A numeric \link{matrix} of counts or a \linkS4class{SingleCellExperiment} returned from \link{celdaGridSearch} with the matrix located in the assay slot under \code{useAssay}. Rows represent features and columns represent cells. Must contain "celda_grid_search" slot in \code{metadata(x)} if \code{x} is a \linkS4class{SingleCellExperiment} object.} \item{...}{Ignored. Placeholder to prevent check warning.} \item{useAssay}{A string specifying which \link{assay} slot to use if \code{x} is a \linkS4class{SingleCellExperiment} object. Default "counts".} \item{altExpName}{The name for the \link{altExp} slot to use. Default "featureSubset".} \item{resample}{Integer. The number of times to resample the counts matrix for evaluating perplexity. Default 5.} \item{seed}{Integer. Passed to \link[withr]{with_seed}. For reproducibility, a default value of 12345 is used. If NULL, no calls to \link[withr]{with_seed} are made.} \item{celdaList}{Object of class 'celdaList'. Used only if \code{x} is a matrix object.} } \value{ A \linkS4class{SingleCellExperiment} object or \code{celdaList} object with a \code{perplexity} property, detailing the perplexity of all K/L combinations that appeared in the celdaList's models. } \description{ Calculates the perplexity of each model's cluster assignments given the provided countMatrix, as well as resamplings of that count matrix, providing a distribution of perplexities and a better sense of the quality of a given K/L choice. } \examples{ data(sceCeldaCGGridSearch) sce <- resamplePerplexity(sceCeldaCGGridSearch) plotGridSearchPerplexity(sce) data(celdaCGSim, celdaCGGridSearchRes) celdaCGGridSearchRes <- resamplePerplexity( celdaCGSim$counts, celdaCGGridSearchRes ) plotGridSearchPerplexity(celdaCGGridSearchRes) }