# alpine  (the [Sassolungo](https://en.wikipedia.org/wiki/Langkofel) mountain in the Dolomites) *alpine* is an R/Bioconductor package for modeling and correcting fragment sequence bias for RNA-seq transcript abundance estimation. In particular, it is the first method of its kind to take into account sample-specific dependence of RNA-seq fragments on their GC content. An example workflow can be found in `vignettes/alpine.Rmd`, or by typing: ```{r} vignette("alpine") ``` *alpine* is designed for un-stranded paired-end RNA-seq data. A paper explaining the *alpine* methods was published in December 2016: <http://dx.doi.org/10.1038/nbt.3682>