\name{DEsubs} \alias{DEsubs} \title{Default run of DEsubs} \usage{ DEsubs(org, mRNAexpr, mRNAnomenclature, pathways, DEtool, DEpar, CORtool, CORpar, subpathwayType, rankedList, verbose) } \arguments{ \item{org}{Organism identifier ('hsa', 'mmu', 'rno', 'sce', 'ath', 'dme', 'dre')} \item{mRNAexpr}{RNA-seq expression data in the form of either a matrix or a filename of a text file stored in the 'User' directory.} \item{mRNAnomenclature}{mRNAnomenclature ('entrezgene', 'ensembl_gene_id', 'ensembl_transcript_id', 'ensembl_peptide_id', 'hgnc_id', 'hgnc_symbol', 'hgnc_transcript_name', 'refseq_mrna', 'refseq_peptide')} \item{pathways}{Pathway type ('All', 'Non-Metabolic', 'Metabolic')} \item{DEtool}{DEG analysis tool selection for NodeRule ('edgeR', 'DESeq', 'EBSeq', 'NBPSeq', 'voom+limma', 'vst+limma', 'TSPM')} \item{DEpar}{DE analysis tools Q-value threshold of NodeRule (default: DEGpar = 0.05)} \item{CORtool}{ Correlation measure selection for EdgeRule ('pearson', 'kendall', 'spearman') } \item{CORpar}{ Correlation measure threshold of EdgeRule (default: CORpar = 0.6) } \item{subpathwayType}{ Subpathway extraction type selection (get all availiable options from \link{subpathwayTypes}) } \item{rankedList}{ A named vector of genes and their corresponding significance of differential expression in the form of a Q-value. If the argument is not null, no DEtool is used for differential expression analysis.} \item{verbose}{ TRUE to display informative messages, FALSE to hide. } } \value{ A list used as input in \code{\link{geneVisualization}}, \code{\link{subpathwayVisualization}}, \code{\link{subpathwayToGraph}}, \code{\link{organismVisualization}} } \description{ Default run of DEsubs } \details{ \itemize{ \item{ Class vector needed values 1 and 2 for each class (eg. control and disease samples). } \item{ DEpar should be less than 0.05 in order to return statistically significant DEGs.} \item{ Higher CORpar values result in stricter correlation criteria, i.e. less acceptable interactions. } } } \examples{ load(system.file('extdata', 'data.RData', package='DEsubs')) DEsubs.run <- DEsubs( org='hsa', mRNAexpr=mRNAexpr, mRNAnomenclature='entrezgene', pathways='All', DEtool=NULL, DEpar=0.05, CORtool='pearson', CORpar=0.6, subpathwayType=NULL, rankedList=rankedList) }