Package: COTAN Type: Package Title: COexpression Tables ANalysis Version: 1.2.0 Authors@R: c( person("Galfrè", "Silvia Giulia",email = "silvia.galfre@uniroma1.it", role=c("aut","cre"),comment = c(ORCID = "0000-0002-2770-0344")), person("Morandin","Francesco", email = "francesco.morandin@unipr.it", role = "aut",comment = c(ORCID = "0000-0002-2022-2300")), person("Pietrosanto","Marco", role = "aut",comment = c(ORCID = "0000-0001-5129-6065")), person("Cremisi","Federico", role = "aut",comment = c(ORCID = "0000-0003-4925-2703")), person("Helmer-Citterich","Manuela", role = "aut",comment = c(ORCID = "0000-0001-9530-7504"))) Description: Statistical and computational method to analyze the co-expression of gene pairs at single cell level. It provides the foundation for single-cell gene interactome analysis. The basic idea is studying the zero UMI counts' distribution instead of focusing on positive counts; this is done with a generalized contingency tables framework. COTAN can effectively assess the correlated or anti-correlated expression of gene pairs. It provides a numerical index related to the correlation and an approximate p-value for the associated independence test. COTAN can also evaluate whether single genes are differentially expressed, scoring them with a newly defined global differentiation index. Moreover, this approach provides ways to plot and cluster genes according to their co-expression pattern with other genes, effectively helping the study of gene interactions and becoming a new tool to identify cell-identity marker genes. URL: https://github.com/seriph78/COTAN BugReports: https://github.com/seriph78/COTAN/issues Depends: R (>= 4.1) License: GPL-3 Encoding: UTF-8 RoxygenNote: 7.2.0 Imports: dplyr, methods, grDevices, Matrix, ggplot2, ggrepel, stats, parallel, tibble, tidyr, irlba, ComplexHeatmap, circlize, grid, scales, utils, rlang, Rfast Suggests: testthat (>= 3.0.0), spelling, knitr, data.table, R.utils, tidyverse, rmarkdown, htmlwidgets, MASS, factoextra, Rtsne, plotly, dendextend, BiocStyle, cowplot Config/testthat/edition: 3 Language: en-US biocViews: SystemsBiology, Transcriptomics, GeneExpression, SingleCell VignetteBuilder: knitr LazyData: false