### ----------------------------------------------------------------- ### Fetch the CNE coordinates from SQL and compute the densities ### Exported! CNEDensity <- function(dbName, tableName, chr, start, end, whichAssembly=c("first", "second"), windowSize=300, minLength=NULL){ .CNEDensityInternal(dbName=dbName, tableName=tableName, whichAssembly=whichAssembly, chr=chr, start=start, end=end, windowSize=windowSize, minLength=minLength) } .CNEDensityInternal <- function(dbName, tableName, whichAssembly=c("first","second"), chr, start, end, windowSize, minLength=NULL){ nrGraphs <- 1 CNEstart <- start CNEend <- end stopifnot(length(CNEstart) == 1L) stopifnot(length(CNEend) == 1L) # This is the pipeline of doing the density plot # The windowSize is in kb. whichAssembly <- match.arg(whichAssembly) windowSize <- as.integer(windowSize) * 1000 # make things easier if(windowSize %% 2 == 0) windowSize <- windowSize - 1L context_start <- as.integer(max(CNEstart - (windowSize-1L)/2, 1)) context_end <- as.integer(CNEend + (windowSize-1)/2) rangesPair <- readCNERangesFromSQLite(dbName, tableName, chr, context_start, context_end, whichAssembly, minLength) ## When no CNEs are returned if(length(rangesPair) == 0L){ ans <- GRanges(seqnames=chr, ranges=IRanges(start=context_start, end=context_end), strand="*", score=0) return(ans) } if(whichAssembly == "first"){ ranges <- first(rangesPair) }else if(whichAssembly == "second"){ ranges <- second(rangesPair) } # Implement get_cne_ranges_in_region_partitioned_by_other_chr later!!! ranges <- reduce(ranges) covAll <- coverage(ranges, width=context_end) runMeanAll <- suppressWarnings(runmean(covAll, k=windowSize, "constant")) ans <- as(runMeanAll, "GRanges") ans$score <- ans$score * 100 return(ans) }