## CCPlotR ### A small R package for visualising results from tools that predict cell-cell interactions from scRNA-seq data This R package makes generic plots that can be used to visualise results from multiple tools such as Liana, CellPhoneDB, NATMI etc. All it requires as input is a dataframe with columns `source`, `target`, `ligand`, `receptor` and `score`. It should look something like this: | source | target | ligand | receptor | score | | ------ | ------ | -------- | -------- | ----- | | B | CD8 T | HLA-DQA1 | LAG3 | 7.22 | | B | CD8 T | HLA-DRA | LAG3 | 5.59 | | CD8 T | NK | B2M | KIR2DL3 | 5.52 | | B | CD8 T | HLA-DQA2 | LAG3 | 5.41 | | NK | B | LGALS1 | CD69 | 4.15 | | B | CD8 T | ICAM3 | ITGAL | 2.34 | For some of the plots, there is an option to also show the expression of the ligands and receptors in each cell type. For those plots, a second dataframe is required, which holds the mean expression values for each gene in each cell type and should look something like this: | cell_type | gene | mean_exp | | --------- | ------ | -------- | | B | ACTR2 | 0.363 | | B | ADA | 0.0170 | | B | ADAM10 | 0.0833 | | B | ADAM28 | 0.487 | | B | ADCY7 | 0.0336 | | B | ADRB2 | 0.0178 | The package comes with toy datasets (`toy_data`, `toy_exp`) which you can see for examples of input data. -------------------- ### Installation The R package can be installed by running: ```R # install development version devtools::install_github("Sarah145/CCPlotR") # or install from Bioconductor if (!requireNamespace("BiocManager", quietly = TRUE)) { install.packages("BiocManager") } BiocManager::install("CCPlotR") ``` ### Plot types The package contains functions for making six types of plots: `cc_heatmap`, `cc_dotplot`, `cc_network`, `cc_circos`, `cc_arrow` and `cc_sigmoid`. Below are some examples of each plot type. #### Heatmaps (`cc_heatmap`) This function can generate heatmaps in four different styles. Option A just displays the total number of interactions between each pair of cell types and option B shows the ligands, receptors and cell types involved in each interaction as well as their score. For option B, only a small portion of top interactions are shown to avoid cluttering the plot. There is also an option to generate heatmaps in the style of popular cell-cell interaction prediction tools such as CellPhoneDB and Liana. ```R library(CCPlotR) data(toy_data, toy_exp, package = 'CCPlotR') cc_heatmap(toy_data) cc_heatmap(toy_data, option = 'B', n_top_ints = 10) cc_heatmap(toy_data, option = 'CellPhoneDB') ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/heatmaps.png"> #### Dotplots (`cc_dotplot`) This function can generate dotplots in four different styles. Option A just displays the total number of interactions between each pair of cell types and option B shows the ligands, receptors and cell types involved in each interaction as well as their score. For option B, only a small portion of top interactions are shown to avoid cluttering the plot. There is also an option to generate dotplots in the style of popular cell-cell interaction prediction tools such as CellPhoneDB and Liana. ```R cc_dotplot(toy_data) cc_dotplot(toy_data, option = 'B', n_top_ints = 10) cc_dotplot(toy_data, option = 'Liana', n_top_ints = 15) ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/dotplots.png"> #### Network (`cc_network`) This function can generate two different types of network plots. In option A, the nodes are cell types and the weight of the edges corresponds to the total number of interactions between a given pair of cell types. In option B, the nodes are ligand and receptor genes, coloured by which cell type is expressing them. For option B, only a small portion of top interactions are shown to avoid cluttering the plot. ```R cc_network(toy_data) cc_network(toy_data, colours = c('orange', 'cornflowerblue', 'hotpink'), option = 'B') ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/networks.png"> #### Circos plot (`cc_circos`) This function can generate three different types of circos plots. Option A generates a circos plot where the width of the links represents the total number of interactions between each pair of cell types. Option B generates a circos plot showing the ligands, receptors and cell types involved in the top portion of interactions. Option C expands on option B by also showing the mean expression of the ligand and receptor genes in each cell type. In options B and C, the weight of the links represents the score of the interaction. ```R cc_circos(toy_data) cc_circos(toy_data, option = 'B', n_top_ints = 10) cc_circos(toy_data, option = 'C', n_top_ints = 15, exp_df = toy_exp, cell_cols = c(`B` = 'hotpink', `NK` = 'orange', `CD8 T` = 'cornflowerblue'), palette = 'PuRd') ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/circos_plots.png"> #### Paired arrow plot (`cc_arrow`) This function generates plots showing the interactions between a given pair of cell types. Option A just shows which ligands/receptors are interacting between a pair of cell types and option B also shows the expression of the ligand/receptor genes in each cell type. In both options, the weight of the arrow represents the score of the interaction. ```R cc_arrow(toy_data, cell_types = c('B', 'CD8 T'), colours = c(`B` = 'hotpink', `CD8 T` = 'orange')) cc_arrow(toy_data, cell_types = c('NK', 'CD8 T'), option = 'B', exp_df = toy_exp, n_top_ints = 10, palette = 'OrRd') ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/arrow_plots.png"> #### Sigmoid plot (`cc_sigmoid`) This function plots a portion of interactions using the `geom_sigmoid` function from the `ggbump` R package to connect ligands in sender cells to receptors in receiver cells. ```R cc_sigmoid(toy_data) ``` <img src="https://github.com/Sarah145/CCPlotR/blob/main/plots/sigmoid.png">