Name Mode Size
R 040000
inst 040000
man 040000
tests 040000
vignettes 040000
.Rbuildignore 100644 0 kb
.gitignore 100644 0 kb
DESCRIPTION 100644 1 kb
NAMESPACE 100644 1 kb 100644 2 kb 100644 12 kb
Introduction ============ This package produces AnVIL workspaces from R packages. An example uses the new [Gen3]( package as a basis for the [Bioconductor-Package-Gen3]( workspace (permission to access this workspace is required, but there are no restrictions on granting permission). Package installation -------------------- If necessary, install the AnVILPublish library if (!"AnVILPublish" %in% rownames(installed.packages())) BiocManager::install("Bioconductor/AnVILPublish") There are only a small number of functions in the package; it is likely best practice to invoke these using `AnVILPublish::...()` rather than attaching the package to the search path. The `gcloud` SDK ---------------- It is necessary to have the [gcloud SDK]( available to copy notebook files to the workspace. Test availability with AnVIL::gcloud_exists() and verify that the account and project are appropriate (consistent with AnVIL credentials) for use with AnVIL AnVIL::gcloud_account() AnVIL::gcloud_project() Note that these be used to set, as well as interrogate, the acount and project. `notedown` software ------------------- Conversion of .Rmd vignettes to .ipynb notebooks uses [notedown]( python software. It must be available from within *R*, e.g., system2("notedown", "--version") Creating or updating workspaces =============================== **CAUTION** updating an existing workspace will replace existing content in a way that cannot be undone – you will lose content! Workspace creation or update uses information from the DESCRIPTION file, and from the YAML metadata at the top of vignettes. It is therefore worth-while to make sure this information is accurate. In the DESCRIPTION file, the Title, Version, Authors@R (preferred) or Author / Maintainer fields, Description, and License fields are used. In vignettes, the title: and author: name: fields are used; the abstract is a good candidate for future inclusion. From package source ------------------- The one-stop route is to create a workspace from the package source (e.g., github checkout) directory use `as_workspace()`. AnVILPublish::as_workspace( "path/to/package", "bioconductor-rpci-anvil", # i.e., billing account create = TRUE # use update = TRUE for an existing workspace ) Use `create = TRUE` to create a new workspace. Use `update = TRUE` to update (and potentially overwrite) an existing workspace. One of `create` and `update` must be TRUE. The command illustrated above does not specify the `name =` argument, so creates or updates a workspace `"Bioconductor-Package-<pkgname>`, where `<pkgname>` is the name of the package read from the DESCRIPTION file; provide an explict name to create or update an arbitrary workspace. `AnVILPublish::as_workspace()` invokes `as_notebook()` and `add_user()`, so these steps do not need to be performed ‘by hand’. From collections of Rmd files ----------------------------- Some *R* resources, e.g., \[bookdown\]\[\] sites, are not in packages. These can be processed to tow workspaces with minor modifications. 1. Add a standard DESCRIPTION file (e.g., `use_this::use_description()`) to the directory containing the `.Rmd` files. 2. Use the `Package:` field to provide a one-word identifier (e.g., `Package: Bioc2020_CNV`) for your material. Add a key-value pair `Type: Workshop` or similar. The `Pacakge:` and `Type:` fields will will be used to create the workspace name as, in the example here, `Bioconductor-Workshop-Bioc2020_CNV`. 3. Add a ‘yaml’ chunk to the top of each .Rmd file, if not already present, including the title and (optionally) name information, e.g., --- title: "01. Introduction to the workshop" author: - name: Iman Author - name: Imanother Author --- Publish the resources with AnVILPublish::as_workspace( "path/to/directory", # directory containing DESCRIPTION file "bioconductor-rpci-anvil", create = TRUE ) Updating notebooks or workspace permissions =========================================== These steps are performed automatically by `as_workspace()`, but may be useful when developing a new workspace or revising existing workspaces. Updating workspace notebooks from vignettes ------------------------------------------- Transforming vignettes to notebooks may require several iterations, and is available as a separate operation. Use `update = FALSE` to create local copies for preview. AnVIL::Publish::as_notebook( "paths/to/files.Rmd", "bioconductor-rpci-anvil", # i.e., billing account "Bioconductor-Package-Foo", # Workspace name update = FALSE # make notebooks, but do not update workspace ) The vignette transformation process has several limitations. Only `.Rmd` vignettes are supported. Currently, the vignette is transformed first to a markdown document using the `rmarkdown` command `render(..., md_document())`. The markdown document is then translated to python notebook using `notedown`. It is likely that some of the limitations of vignette rendering can be reduced. Adding user access credentiials to share the notebook ----------------------------------------------------- The `"Bioconductor_User"` group can be added to the entities that can see the workspace. AnVIL users wishing to view the worksppace should be added to the `Bioconductor_User` group, rather than to the workspace directly. To add the user group, use AnVILPublish::add_user( "bioconductor-rpci-anvil", "Bioconductor-Package-Foo" ) Vignette and .Rmd best practices ================================ Orientation ----------- `.Rmd` files need to be converted to jupyter notebooks. Currently there is not an ‘ideal’ solution, with details listed in the ‘Additional notes…’ section. Consequently, there are ‘best practices’ that lead to results that are more likely to be satisfactory, as outlined here. Best practices -------------- 1. For packages, make sure the DESCRIPTION file is complete. Use the `Authors@R` notation for fully specifying authors. Add a `Date:` field indicating date of last modification. Follow other Bioconductor best practices, e.g., using and incrementing appropriate version numbers. 2. For collections of vignettes not in a package (e.g., a bookdown folder), add a DESCRIPTION file at the top level. An example is Package: BCC2020 Type: Workshop Title: R / Bioconductor in the AnVIL Cloud Version: 1.0.0 Authors@R: c(person( given = "Martin", family = "Morgan", role = c("aut", "cre"), email = "", comment = c(ORCID = "0000-0002-5874-8148") ), person("Nitesh", "Turaga", role = "ctb"), person("Lori", "Shepherd", role = "ctb")) Description: This book contains material for a 2 1/2 hour course offered at the Bioinformatics Community Conference 2020. Bioconductor provides more than 1900 R packages for the analysis and comprehension of high-throughput genomic data. Most users install and run Bioconductor on a personal computer or perhaps use an academic cluster. Cloud-based solutions are increasing appealing, removing the headaches of local installation while providing access to (a) better, scalable computing resources; and (b) large-scale 'consortium' and other reference data sets. This session introduces the AnVIL cloud computing environment. We cover use of the cloud as a replacement to desktop-style computing; integrating workflows for 'upstream' processing of large data resources with interactive 'downstream' analysis and comprehension, using Human Cell Atlas single-cell datasets as an example; and querying cloud-based consortium data for integration with a users own data sets. License: CC-BY Date: 2020-07-17 Encoding: UTF-8 LazyData: true Roxygen: list(markdown = TRUE) RoxygenNote: 7.1.1 The `Type` and `Package` fields are used to construct the second and third elements of the workspace name (in this case, `Bioconductor-Workshop-BCC2020`). `Title`, `Version`, `Authors@R`, `Description`, `License`, and `Date` fields are used to construct the DASHBOARD page. 3. Start each vignette with ‘yaml’ containing essential metadata about the document – title and author(s). Include other information if desired, e.g., abstract, (static) date of last modification. 4. Use a file naming system AND a yaml `title` field that sorts files into the order in which the document content is to be presented, e.g., using file names `01-Setup.Rmd`, `02-...` and titles (in the yaml) `title: "01 Setup"`, … Naming both files and titles in this way provides some chance that the Rmd files are presented, or can be made to be presented, sensibly across the Bioconductor package landing page and Workspace / NOTEBOOK interface. 5. All code chunks, regardless of annotations such as `eval = FALSE` or `echo = FALSE` are converted to visible, evaluated cells in jupyter notebooks. Replace code chunks that you do not wish the user to evaluate with HTML tags `<pre></pre>`. 6. Although both Rmarkdown and python notebooks support code chunks in multiple languages, there is no support for this in the conversion procdess – all cells are presented as *R* code. Additional notes on .Rmd conversion ----------------------------------- The current state of affairs with respect to notebook conversion is imperfect. Conversion is currently a two-step process: Rmarkdown to markdown, and markdown to ipynb. - The conversion from Rmarkdown to markdown is currently accomplished with knitr::opts_chunk$set(eval=FALSE) rmarkdown::render(..., md_document()) to create a markdown document from the `.Rmd` source. This correctly processes the markdown content, including yaml metadata, but renders all code chunks identically. Using other knitr options may allow, e.g., conditional inclusion of code chunks. - Use [`notedown`]( to convert from markdown to jupyter notebook, adding metadata to indicate that the notebook has an *R* kernel. Here are some notes on alternative solutions. - [`jupytext`]( (version 1.5.1) but has difficulty with some markdown. For instance, reference-style links `[foo][1]` are only rendered correctly when the reference is in the same code chunk as the link. It is under active development and may mature into a possible alternative. - `pandoc` (version 2.10.1) provides a one-step convertion from `.Rmd` to .`ipynb`, but code chunks are rendered as pre-formatted text rather than evaluable cell. - [`notedown`]( (version 1.5.1) also provides one-step conversion, but does not exclude yaml from vignettes. The project has not had commits for several years, and has several open issues. Session info ============ sessionInfo() ## R version 4.0.2 (2020-06-22) ## Platform: x86_64-pc-linux-gnu (64-bit) ## Running under: Ubuntu 20.04.1 LTS ## ## Matrix products: default ## BLAS: /usr/lib/x86_64-linux-gnu/blas/ ## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/ ## ## locale: ## [1] LC_CTYPE=C.UTF-8 LC_NUMERIC=C LC_TIME=C.UTF-8 ## [4] LC_COLLATE=C.UTF-8 LC_MONETARY=C.UTF-8 LC_MESSAGES=C.UTF-8 ## [7] LC_PAPER=C.UTF-8 LC_NAME=C LC_ADDRESS=C ## [10] LC_TELEPHONE=C LC_MEASUREMENT=C.UTF-8 LC_IDENTIFICATION=C ## ## attached base packages: ## [1] stats graphics grDevices utils datasets methods base ## ## loaded via a namespace (and not attached): ## [1] compiler_4.0.2 magrittr_1.5 tools_4.0.2 htmltools_0.5.0 ## [5] yaml_2.2.1 stringi_1.4.6 rmarkdown_2.3 knitr_1.29 ## [9] stringr_1.4.0 xfun_0.16 digest_0.6.25 rlang_0.4.7 ## [13] evaluate_0.14